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brassica 95k est microarray  (Cogenics Inc)

 
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    Cogenics Inc brassica 95k est microarray
    Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and <t>microarray</t> data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.
    Brassica 95k Est Microarray, supplied by Cogenics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brassica+95k+est+microarray/brassica+95k+est+microarray/pmc04094393-111-15-27
    Average 90 stars, based on 1 article reviews
    brassica 95k est microarray - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling"

    Article Title: Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0101914

    Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.
    Figure Legend Snippet: Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.

    Techniques Used: Expressing, Gene Expression, Quantitative RT-PCR, Microarray

    Related Articles

    Microarray:

    Article Title: Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling
    Article Snippet: .. To identify genes responsive to heat stress in oilseed rape at the seed-filling stage, a Brassica 95k EST microarray (jointly developed by the John Innes Centre and Cogenics ) was used to profile the transcripts from both the SW and seeds separated from 20 DAF siliques. ..



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    brassica 95k est microarray  (Agilent technologies)
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    Agilent technologies brassica 95k est microarray
    Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and <t>microarray</t> data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.
    Brassica 95k Est Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brassica+95k+est+microarray/pmc04094393-301-23-22
    Average 90 stars, based on 1 article reviews
    brassica 95k est microarray - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    brassica 95k est microarray  (Cogenics Inc)
    90
    Cogenics Inc brassica 95k est microarray
    Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and <t>microarray</t> data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.
    Brassica 95k Est Microarray, supplied by Cogenics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brassica+95k+est+microarray/brassica+95k+est+microarray/pmc04094393-111-15-27
    Average 90 stars, based on 1 article reviews
    brassica 95k est microarray - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.

    Journal: PLoS ONE

    Article Title: Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling

    doi: 10.1371/journal.pone.0101914

    Figure Lengend Snippet: Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.

    Article Snippet: Total RNA was extracted using a cetyltrimethylammonium bromide extraction method and assessed by spectrophotometry and bioanalysis before proceeding to analysis with the Agilent Brassica 95k EST Microarray , developed by the John Innes Centre in collaboration with JCVI (J. Craig Venter Institute) and Cogenics. cDNA synthesis, labeling, hybridization, washing, scanning and data extraction were performed using established procedures for the analysis of eukaryotic RNA by the Cogenics Microarray Core Facility (Morrisville, NC, US).

    Techniques: Expressing, Quantitative RT-PCR, Microarray

    Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.

    Journal: PLoS ONE

    Article Title: Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling

    doi: 10.1371/journal.pone.0101914

    Figure Lengend Snippet: Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.

    Article Snippet: To identify genes responsive to heat stress in oilseed rape at the seed-filling stage, a Brassica 95k EST microarray (jointly developed by the John Innes Centre and Cogenics ) was used to profile the transcripts from both the SW and seeds separated from 20 DAF siliques.

    Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Microarray